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M. R. Taware ¹, V. M. Gholve ¹, S. S. Wagh ¹, D. P. Kuldhar ¹, D. V. Pawar ¹, A. A. Chavan ¹

Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, Prabhani (M.S.), IN

Abstract

Eight culture media, eight temperature levels, nine pH levels, seven carbon and six nitrogen sources tested exhibited better growth of Alternaria carthami. Results revealed that Potato dextrose agar gave significantly highest growth (90.00 mm), followed by Potato malt agar (84.16 mm) with excellent sporulation. Temperature levels indicated that highest mean mycelial growth (85.66 mm) was recorded at 30°C followed by 25°C (83.83 mm) and 20°C (66.33 mm). However, maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent sporulation, followed by at pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively. The carbon sources exhibited varied radial mycelial growth and sporulation of the test pathogen. However, highest radial mycelial growth (86.00 mm) and excellent sporulation was recorded on glucose, followed by on maltose (82.83 mm) and starch (80.33 mm) with excellent sporulation. Nitrogen sources resulted highest radial mycelial growth (82.55 mm) and excellent sporulation on potassium nitrate, followed by on peptone (75.83 mm) with good sporulation. Least radial mycelial growth (19.00 mm) was recorded on urea with poor sporulation.

Keywords

Alternaria Carthami, Mycelial Growth, Carbon And Nitrogen Sources

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V. M. Gholve ¹, M. R. Taware ¹, S. S. Wagh ¹

Affiliations
1 Department of Plant Pathology, Vasantrao Naik Marathwada Krishi Vidyapeeth, PARBHANI (M.S.), IN

Abstract

All eight culture media tested encouraged better growth of Alternaria carthami. However, Potato dextrose agar gave significantly highest growth (90.00 mm). The second and third best culture media found were Potato malt agar (84.16 mm) and Yeast manitol agar (73.33 mm). Rest of the culture media recorded good amount of mycelial growth in the range of 41.66 mm (Yeast extract agar) to 69.16 mm (Malt extract agar). Colony growth was circular, cottony, grayishblack or olivaceous-black coloured with fair to excellent sporulation. The mean colony growth recorded with all the different temperature ranged from 5.66 mm at 5°C to 85.66 mm at 30°C. However, significantly highest mean mycelial growth (85.66 mm) was recorded at 30°C with excellent (++++) sporulation. The second and third best temperature found were 25°C (83.83 mm) and 20°C (66.33 mm). The mean colony growth recorded with all the pH values ranged from 30.50 mm at pH 4.0 to 85.83 mm at pH 6.5. However, significantly maximum mean mycelial growth (85.83 mm) was recorded at pH 6.5 with excellent (++++) sporulation. The second and third best pH values found were pH 6 (82.00 mm) and pH 7 (70.33 mm) with excellent and good sporulation, respectively.

Keywords

Culture Media, Temperature, PH, A. carthami, Growth, Sporulation.

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